Please see examples for a detailed explanation of this calculator. This calculator calculates for the percent recovery of the spike. The required values are as given in the table. The picture illustrates two cases where in one case no solvent make-up volume is used and the other case where solvent is used to make-up the volume to a pre-determined volume mark. In either case the total final volume is the value used in the calculations. The picture is for illustration purposes only, the final solution should be homogeneous. Check out also the Analytical Spike Addition Calculator and the Analytical Spike Addition Calculator with Solvent Addition.